Partially purified fraction (PPF) antigen from adultFasciola Giganticafor the serodiagnosis of human fasioliasis using Dot-ELISA technique
نویسندگان
چکیده
منابع مشابه
Detection of anti-Haemonchus contortus antibodies in sheep by dot-ELISA with immunoaffinity purified fraction of ES antigen during prepatency.
Haemonchosis is a very common disease in small ruminants caused by H. contortus, a blood sucking parasite causing anaemia that may be fatal particularly to young animals. Therefore, detection of the infection during prepatent period is important for early treatment. Excretory-Secretory (ES) protein of H. contortus was purified through immunoaffinity chromatography. Dot -ELISA was performed with...
متن کاملDiagnosis of Eimeriosis in Cattle by ELISA Using Partially Purified Antigen
Chromatographic analysis of Eimeria sp. crude antigen was done by Sephacryl S-200 column. Four fractions were isolated by this approach. Comparative evaluation of the immunogenic binding activities of isolated fractions by ELISA proved the potency of fraction number one (F1) over the other three ones. Characterization of the isolated fraction (F1) by SDS polyacrylamide gel electrophoresis showe...
متن کاملA dot-ELISA test using a Gnathostoma spinigerum recombinant matrix metalloproteinase protein for the serodiagnosis of human gnathostomiasis.
Gnathostomiasis caused by Gnathostoma spinigerum, is a hazardous food- borne helminthic zoonosis, and is endemic especially in developing countries in Asia. Definitive diagnosis, relying upon identification of worms from human tissues or body, is rarely accomplished. Consequently, sensitive supporting tools such as serological tests have been used widely. But these methods are time con- suming,...
متن کاملImmunodiagnosis of Human Fascioliasis by Elisa Using Excretory–Secretory Products as Antigen
Background: Different immunological methods are used for the serodiagnosis of Fasciola hepatica infections, however, they have limited specificity and/or sensitivity.Objective: To improve the specificity of ELISA using a partially modified method for preparation of excretory-secretory (ES) antigens.Methods: Studied groups were as follows: 75 patients with parasitological evidence of fascioliasi...
متن کاملComparison of Dot-ELISA and Sandwich ELISA Diagnostic Tests in Detection of Human Hydatidosis
Hydatidosis, caused by the larval stage of Echinococcus granulosus, is one of the most important zoonosis with worldwide distribution. As its diagnosis by clinical symptoms and scanning alone is difficult and confusing, we designed the present study to achieve a sensitive and simple diagnostic method for epidemiological studies. Sera (250 samples) were collected from 90 cases of hydatidosis pro...
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ژورنال
عنوان ژورنال: Annals of Saudi Medicine
سال: 2004
ISSN: 0256-4947,0975-4466
DOI: 10.5144/0256-4947.2004.18